Chronic inflammation, neutrophil activity, and autoreactivity splits long COVID

Abstract:

While immunologic correlates of COVID-19 have been widely reported, their associations with post-acute sequelae of COVID-19 (PASC) remain less clear. Due to the wide array of PASC presentations, understanding if specific disease features associate with discrete immune processes and therapeutic opportunities is important.

Here we profile patients in the recovery phase of COVID-19 via proteomics screening and machine learning to find signatures of ongoing antiviral B cell development, immune-mediated fibrosis, and markers of cell death in PASC patients but not in controls with uncomplicated recovery. Plasma and immune cell profiling further allow the stratification of PASC into inflammatory and non-inflammatory types.

Inflammatory PASC, identifiable through a refined set of 12 blood markers, displays evidence of ongoing neutrophil activity, B cell memory alterations, and building autoreactivity more than a year post COVID-19. Our work thus helps refine PASC categorization to aid in both therapeutic targeting and epidemiological investigation of PASC.

Source: Woodruff MC, Bonham KS, Anam FA, Walker TA, Faliti CE, Ishii Y, Kaminski CY, Ruunstrom MC, Cooper KR, Truong AD, Dixit AN, Han JE, Ramonell RP, Haddad NS, Rudolph ME, Yalavarthi S, Betin V, Natoli T, Navaz S, Jenks SA, Zuo Y, Knight JS, Khosroshahi A, Lee FE, Sanz I. Chronic inflammation, neutrophil activity, and autoreactivity splits long COVID. Nat Commun. 2023 Jul 14;14(1):4201. doi: 10.1038/s41467-023-40012-7. PMID: 37452024; PMCID: PMC10349085. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10349085/ (Full text)

The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling

Abstract:

Background: COVID-19 is characterized by a heterogeneous clinical presentation, ranging from mild symptoms to severe courses of disease. 9-20% of hospitalized patients with severe lung disease die from COVID-19 and a substantial number of survivors develop long-COVID. Our objective was to provide comprehensive insights into the pathophysiology of severe COVID-19 and to identify liquid biomarkers for disease severity and therapy response.

Methods: We studied a total of 85 lungs (n = 31 COVID autopsy samples; n = 7 influenza A autopsy samples; n = 18 interstitial lung disease explants; n = 24 healthy controls) using the highest resolution Synchrotron radiation-based hierarchical phase-contrast tomography, scanning electron microscopy of microvascular corrosion casts, immunohistochemistry, matrix-assisted laser desorption ionization mass spectrometry imaging, and analysis of mRNA expression and biological pathways. Plasma samples from all disease groups were used for liquid biomarker determination using ELISA. The anatomic/molecular data were analyzed as a function of patients’ hospitalization time.

Findings: The observed patchy/mosaic appearance of COVID-19 in conventional lung imaging resulted from microvascular occlusion and secondary lobular ischemia. The length of hospitalization was associated with increased intussusceptive angiogenesis. This was associated with enhanced angiogenic, and fibrotic gene expression demonstrated by molecular profiling and metabolomic analysis. Increased plasma fibrosis markers correlated with their pulmonary tissue transcript levels and predicted disease severity. Plasma analysis confirmed distinct fibrosis biomarkers (TSP2, GDF15, IGFBP7, Pro-C3) that predicted the fatal trajectory in COVID-19.

Interpretation: Pulmonary severe COVID-19 is a consequence of secondary lobular microischemia and fibrotic remodelling, resulting in a distinctive form of fibrotic interstitial lung disease that contributes to long-COVID.

Source: Ackermann M, Kamp JC, Werlein C, Walsh CL, Stark H, Prade V, Surabattula R, Wagner WL, Disney C, Bodey AJ, Illig T, Leeming DJ, Karsdal MA, Tzankov A, Boor P, Kühnel MP, Länger FP, Verleden SE, Kvasnicka HM, Kreipe HH, Haverich A, Black SM, Walch A, Tafforeau P, Lee PD, Hoeper MM, Welte T, Seeliger B, David S, Schuppan D, Mentzer SJ, Jonigk DD. The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling. EBioMedicine. 2022 Nov;85:104296. doi: 10.1016/j.ebiom.2022.104296. Epub 2022 Oct 4. PMID: 36206625; PMCID: PMC9535314. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535314/ (Full text)

Clinical and radiological outcomes of longCOVID: Is the post-COVID fibrosis common?

Abstract:

Introduction: COVID-19 survivors may take longer to regain full well-being. This study aimed to investigate clinical and functional evaluation and radiologic changes in the third month after COVID-19.

Materials and methods: A total of 126 patients were assessed in the third month for symptoms, pulmonary function, exercise capacity, radiologic imaging, and quality of life after being discharged following COVID-19 treatment. Two radiologists evaluated the initial and follow-up images.

Result: At the third month follow-up visit, the most common persisting symptoms were shortness of breath (32.5%), cough (12.7%), and muscle pain (12.7%). At the follow-up visit, oxygen saturations at rest and after a six min walking test were lower in patients with prior intensive care hospitalization compared to those without (p<0.001, p= 0.004). Computed tomography (CT) scans revealed persisting pulmonary pathologies in 64.6% of patients at the third month follow-up. The most common pathologies on follow-up thoracic CT were fibrotic-like changes in 44.2% and ground-glass opacities (GGO) in 33.3%. Regression analysis unveiled that age [95% confidence interval (CI), 1.01 to 1.15; p= 0.020], male sex (95% CI, 4.06 to 95.3, p<0.001), first CT severity score (95% CI, 1.02 to 1.41, p= 0.028), duration of hospitalization (95% CI, 1.02 to 1.18, p= 0.012), oxygen saturation (95% CI, 0.86 to 0.96, p<0.001) were independent predictors of fibrotic-like changes.

Conclusions: In the third month following COVID-19, the most common symptom was dyspnea, and the most common radiological findings were fibrotic-like changes and GGO. Longer follow-up studies of COVID-19 survivors are needed to observe lasting changes.

Source: Sarıoğlu N, Aksu GD, Çoban H, Bülbül E, Demirpolat G, Arslan AT, Erel F. Clinical and radiological outcomes of longCOVID: Is the post-COVID fibrosis common? Tuberk Toraks. 2023 Mar;71(1):48-57. English. doi: 10.5578/tt.20239907. PMID: 36912409. http://tuberktoraks.org/managete/fu_folder/2023-01/2023-71-1-48-57.pdf (Full text)

Persistent alveolar type 2 dysfunction and lung structural derangement in post-acute COVID-19

Abstract:

SARS-CoV-2 infection can manifest as a wide range of respiratory and systemic symptoms well after the acute phase of infection in over 50% of patients. Key questions remain on the long-term effects of infection on tissue pathology in recovered COVID-19 patients. To address these questions we performed multiplexed imaging of post-mortem lung tissue from 12 individuals who died post-acute COVID-19 (PC) and compare them to lung tissue from patients who died during the acute phase of COVID-19, or patients who died with idiopathic pulmonary fibrosis (IPF), and otherwise healthy lung tissue.

We find evidence of viral presence in the lung up to 359 days after the acute phase of disease, including in patients with negative nasopharyngeal swab tests. The lung of PC patients are characterized by the accumulation of senescent alveolar type 2 cells, fibrosis with hypervascularization of peribronchial areas and alveolar septa, as the most pronounced pathophysiological features. At the cellular level, lung disease of PC patients, while distinct, shares pathological features with the chronic pulmonary disease of IPF. which may help rationalize interventions for PC patients.

Altogether, this study provides an important foundation for the understanding of the long-term effects of SARS-CoV-2 pulmonary infection at the microanatomical, cellular, and molecular level.

Source: André F. RendeiroHiranmayi RavichandranJunbum KimAlain C. BorczukOlivier ElementoRobert E. Schwartz. Persistent alveolar type 2 dysfunction and lung structural derangement in post-acute COVID-19.

Mid-term Follow-Up chest CT findings in recovered COVID-19 patients with residual symptoms

Abstract:

Objectives: More than a year has passed since the initial outbreak of SARS-CoV-2, which caused many hospitalizations worldwide due to COVID-19 pneumonia and its complications. However, there is still a lack of information detailing short- and long-term outcomes of previously hospitalized patients. The purpose of this study is to analyze the most frequent lung CT findings in recovered COVID-19 patients at mid-term follow-ups.

Methods: A total of 407 consecutive COVID-19 patients who were admitted to the XXXX and discharged between February 27, 2020, and June 26, 2020 were recruited into this study. Out of these patients, a subset of 108 patients who presented with residual asthenia and dyspnea at discharge, altered spirometric data, positive lung ultrasound and positive chest X-ray was subsequently selected, and was scheduled to undergo a mid-term chest computer tomography study, which was evaluated for specific lung alterations and morphological patterns.

Results: The most frequently observed lung CT alterations, in order of frequency, were ground glass opacities (81%), linear opacities (74%), bronchiolectases (64,81%), and reticular opacities (63,88%). The most common morphological pattern was the nonspecific interstitial pneumonia pattern (63,88%). Features consistent with pulmonary fibrosis were observed in 32 patients (29,62%).

Conclusions: Our work showed that recovered COVID-19 patients that were hospitalized and that exhibited residual symptoms after discharge had a slow radiological recovery with persistent residual lung alterations.

Advances in knowledge: This slow recovery process should be kept in mind when determining the follow-up phases in order to improve the long-term management of patients affected by COVID-19.

Source: Marchetti F, Izzi N, Donatelli A, Valentini A, Muzic SI, Dore R, Di Sabatino A, Perrone T, Falaschi F, Sabatini U, Ballesio A, Meloni F, Lettieri S, Mojoli F, Perlini S, Novati S, Pagani E, Klersy C, Bruno R, Preda L. Mid-term Follow-Up chest CT findings in recovered COVID-19 patients with residual symptoms. Br J Radiol. 2022 Nov 25:20220012. doi: 10.1259/bjr.20220012. Epub ahead of print. PMID: 36427055.  https://pubmed.ncbi.nlm.nih.gov/36427055/

What Are the Long-term Pulmonary Sequelae of COVID-19 Infection?

The COVID-19 pandemic has been ongoing for almost two years. Over this period, Radiology and other peer-reviewed journals have distributed information regarding the nature of the pandemic with unprecedented speed. Based upon the extensively documented clinical and imaging manifestations of acute COVID-19 infection, expert thoracic imagers have developed imaging categories that classify patterns according to the likelihood that they represent COVID-19 infection(1).

Acute COVID-19 has a somewhat unique appearance amongst viral infections on CT. It manifests as ground-glass opacities and/or consolidation often with a strong peripheral distribution. Also, there are CT findings suggesting that organizing pneumonia is a common pattern of injury. Organizing pneumonia is associated with a wide variety of different infections, however, it appears particularly common with COVID-19(2). However, the long-term pulmonary manifestations of COVID-19 pneumonia (part of so-called “long-COVID”) remain lacking in the literature.

It is important to understand our current knowledge of viral infections and their typical manifestations within the lungs. The long-term sequela of viral pneumonia, in general, vary depending upon two factors: 1) direct injury caused by the viral organisms, and 2) the host’s immune reaction to those organisms. These result in a variety of different patterns of injury, each of which is associated with specific permanent long-term sequela. The histologic manifestations of acute pulmonary viral infections can be divided broadly into two primary patterns: 1) bronchiolitis and inflammation adjacent to airways, and 2) diffuse alveolar damage. On imaging, bronchiolitis and airway inflammation manifest as bronchial wall thickening, centrilobular nodules, and tree-in-bud opacities; whereas diffuse alveolar damage manifests as bilateral ground-glass opacity and/or consolidation.

The long-term effects of these two patterns are also characteristic. Inflammation within and around the airways may induce concentric fibrosis around the bronchioles resulting in airway narrowing or obliteration. This is termed constrictive (or obliterative) bronchiolitis. Development of constrictive bronchiolitis may result in persistent dyspnea after resolution of the acute infection with an associated obstructive defect on pulmonary function tests. Typical CT findings of constrictive bronchiolitis include mosaic attenuation and air trapping, sometimes associated with bronchiectasis. The long-term manifestations of diffuse alveolar damage (DAD), on the other hand, are quite different. Histologically, fibrosis develops 1-2 weeks after the development of acute symptoms. On imaging, this is associated with the development of reticulation and traction bronchiectasis. Over time, usually months, this fibrosis may improve, although residual fibrosis is common(3). This residual fibrosis is often located in the anterior subpleural lung and may be associated with restrictive physiology on pulmonary function testing.

Organizing pneumonia (OP) is particularly common with COVID-19. The clinical and imaging features of OP have been studied (4) mainly in the setting of cryptogenic (idiopathic) disease. Organizing pneumonia is usually a highly steroid-responsive disease with opacities that quickly improve or resolve with treatment, although residual fibrosis may occur. This residual fibrosis often has a pattern that resembles nonspecific interstitial pneumonia with basilar predominant reticulation, traction bronchiectasis, and subpleural sparing(5). It is also important to note that OP and DAD may co-exist with overlapping imaging findings.

Understanding the different patterns of injury associated with viral infections and their long-term sequela is important in putting the long-term effects of COVID-19 infection in context. Han et al(6). were among the first to describe the persistent CT findings of COVID-19 six months after the onset of acute symptoms. In their study, over one-third of patients showed evidence of fibrotic changes.

In this issue of Radiology, Cho and Villacreses and colleagues (7) address these long-term pulmonary manifestations in a prospective study of 100 patients with persistent (>30 days) pulmonary symptoms after an acute COVID-19 infection. One hundred and six healthy controls were also evaluated. The particular emphasis of this investigation was on the presence of air trapping on expiratory CT. The severity of disease among studied patients varied and included outpatients, hospitalized patients, and those requiring admission to the intensive care unit (ICU). Cho and Villacreses et al. discovered that air trapping was present in 58% of patients with post-COVID-19 and had its highest prevalence in the group of patients hospitalized for their infection (73%). Using quantitative analysis, air trapping affected a mean of 25-35% of the lungs in patients with post-COVID-19 depending on the clinical severity of disease compared to 7% in controls (p<.001). The authors did not identify obstructive airways disease on spirometry in any of the groups. This lack of obstruction on spirometry in patients with air trapping is not surprising. In a cohort of soldiers deployed to Iraq and Afghanistan with biopsy-proven constrictive bronchiolitis(8), the majority did not have obstruction on pulmonary function tests. Restriction was present on spirometry in the patients with COVID-19 in the study by Cho and Villacreses et al., specifically in the inpatient and ICU groups. Ground-glass opacity, traction bronchiectasis, and other signs of fibrosis were most frequent in patients admitted to the ICU (94%, 69%, 81% of patients, respectively compared with 36%, 8%, and 3% of outpatients, respectively).

In summary, the study by Cho and Villacreses et al. demonstrates that air trapping on CT is common in patients with persistent symptoms after COVID-19 infection. When considering the long-term pulmonary effects of COVID-19 infection, this is an important finding and may correspond to the development of post-viral constrictive bronchiolitis, an entity seen with other viral infections and in particular, adenovirus infection. Interestingly, the CT findings of acute COVID-19 are not highly airway-centric. Centrilobular nodules and tree-in-bud opacities, reflecting airway-centric inflammation, are not a typical finding of acute COVID-19 infection. Regardless, these results indicate a long-term impact on bronchiolar obstruction. In the study by Cho and Villacreses et al, the presence of ground glass opacity and/or fibrosis on CT were most common in the patients admitted to the ICU and likely correspond to post-OP and/or post-DAD fibrosis.

It is important to note that not all pulmonary fibrosis, including that of the airway and of the parenchyma, is permanent. Collagen may be absorbed for months after the acute insult, thus it is not entirely clear if the abnormalities seen in the current study will be permanent. The median time from COVID-19 diagnosis to the clinic visit for persistent post-COVID-19 symptoms was only 75 days. However, 8 of 9 patients (out of 100 patients total) with imaging more than 200 days from the acute infection had persistent air trapping. Regardless of the imaging findings, the most important question is whether the airway obstruction and/or fibrosis result in clinical symptoms. This paper suggests that airways obstruction and post-OP/DAD fibrosis contribute to persistent symptoms after COVID-19 infection with the contribution of airways disease higher in the outpatients, and the contribution of OP/DAD greater in the patients admitted to ICU. Longer-term studies assessing the clinical and imaging manifestations 1-2 years after the initial infection are needed to fully ascertain the permanent manifestations of post-COVID fibrosis.

Source: Brett M. Elicker. What Are the Long-term Pulmonary Sequelae of COVID-19 Infection? Radiology. Published Online:

Complications of sarcoidosis. Chronic fatigue syndrome

Abstract:

Well-recognised complications are pulmonary fibrosis, cor pulmonale, glaucoma, cataract and nephrocalcinosis causing failure of lungs, heart, vision and kidneys. Less well-recognised is the post-sarcoidosis chronic fatigue syndrome.

The afflicted join sarcoidosis patients’ associations because of their profound symptoms of myalgia, fatigue, sleep reversal and low-spiritedness. The symptoms are out of proportion to the lack of physical signs and the absence of objective evidence of sarcoidosis.

Management includes unremitting sympathy and replenishment of essential neurochemicals.
Comment in: Complications of sarcoidosis. Chronic fatigue syndrome. [Sarcoidosis. 1993]

 

Source: James DG. Complications of sarcoidosis. Chronic fatigue syndrome. Sarcoidosis. 1993 Mar;10(1):1-3. http://www.ncbi.nlm.nih.gov/pubmed/8134708